Calculating coordinates in pixels
add_mic_loc(Spots_df, xres = 0.1625, yres = 0.1625, zres = 0.25, x, y, z)
| Spots_df | a dataframe with spots information and coordinates in microns |
|---|---|
| xres | resolution in microns for the x dimension |
| yres | resolution in microns for the y dimension |
| zres | resolution in microns for the z dimension |
| x | x coordinates in microns |
| y | y coordinates in microns |
| z | z coordinates in microns |
dataframe with three new columns